@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAfAGouxD007zkf9AFHhJZkiq0kr-VRzRCNupzdTems4A
> .
@prefix sub: <
http://purl.org/np/RAfAGouxD007zkf9AFHhJZkiq0kr-VRzRCNupzdTems4A#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_53692
>
rdfs:label
"histopaque" .
sub:_1
rdfs:label
"PBMCs" .
sub:_2
rdfs:label
"PBMCs" .
sub:_3
rdfs:label
"medium" .
sub:step
dce:description
"Aspirate the whitish buffy coat (about 1 ml) (PBMCs) formed in the interphase between histopaque and medium." ;
sp:hasExperimentalOutput
sub:_1
;
a
pplan:Step
;
bs:bioSampleUsed
sub:_2
;
bs:reagentUsed
<
http://purl.obolibrary.org/obo/CHEBI_53692
> ,
sub:_3
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-14T04:55:43.561675"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:9udhhlbrEeungHMTQnSNRA
,
authority:FkKG5lbsEeungQ9zZBkQzA
,
authority:O9mHLlbsEeu0jUvrFwQ_uw
,
authority:uOfBblbrEeusAOM2O7_2zQ
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"Rf7F2wxv1atr20XMvIA+nvMZ7NQ/gn0jRQXkqyRaCIoZ9bub/Acpa64j/130+9X8tSSZ9+bsTKrM4tSo2i2mnjhveDdYPJ9gMewo3buwDLkFW9iVMgAyd3PT+qXftU9+KJSMJcX0A0nxe07nF9VEg5/TigX8a4lzBrjaDPwWt84=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-14T04:55:43.561675"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}