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http://schema.org/contentSize 10641 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/contentUrl https://api.rohub.org/api/ros/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/crate/download/ https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/creator mailto:georgehadib@gmail.com https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/dateCreated 2022-03-22 00:58:15.830962+00:00 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/dateModified 2025-03-05 01:17:02.044283+00:00 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/datePublished 2022-03-22 00:58:15.830962+00:00 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/description Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT. https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/encodingFormat application/ld+json https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/hasPart https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/folders/05168684-1a72-430f-9fab-46996acf9227 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/hasPart https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/folders/39ed57a2-dd8a-4b28-a9a5-02c94f9cfa90 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/hasPart https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/folders/c33c51c0-fc8c-4c89-945b-9e5a9bea7862 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/hasPart https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/folders/e5495675-2f14-47f1-b19d-57d34625f76c https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/identifier https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/license https://choosealicense.com/no-permission/ https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://schema.org/name Raw data for Frei, D. M. et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://w3id.org/ro-id/rohub/model#creation_mode MANUAL https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/wf4ever/ro#ResearchObject https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/wf4ever/roevo#LiveRO https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://schema.org/Dataset https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://w3id.org/ro/earth-science#DataResearchObject https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ http://www.w3.org/1999/02/22-rdf-syntax-ns#type https://w3id.org/ro/terms/earth-science#DataResearchObject https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/ https://www.w3.org/ns/iana/link-relations/relation#cite-as Dominik Michael Frei, University of Bergen, Institute of biomedicine (UiB), Tanja Kögel, and Erlend Hodneland. "Raw data for Frei, D. M. et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer." ROHub. 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M., University of Bergen, Institute of biomedicine, Kögel, T., Hodneland, E. (2016).Raw data for Frei, D. M. et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer [Data set]. Norstore. https://doi.org/10.11582/2016.00001 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://purl.org/dc/terms/rightsHolder Universitet i Bergen, Institutt for biomedisin (UiB, IBM) https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://purl.org/dc/terms/type Image https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/author mailto:georgehadib@gmail.com https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/contentUrl https://archive.sigma2.no/pages/public/datasetDetail.jsf?id=10.11582/2016.00001 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/creator mailto:georgehadib@gmail.com https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/dateCreated 2016-04-22 00:00:00 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/dateModified 2022-03-22 00:58:42.545289+00:00 https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/description Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT. https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/license https://choosealicense.com/no-permission/ https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a/resources/f4520e2b-26af-4854-8c6b-3870468662f3 http://schema.org/name Raw data for Frei, D. M. et al. 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