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@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAhLBnCkTIy1O1RJGNjrqqWs3d9AifkFB5TOV_4bIv1Ig
> .
@prefix sub: <
http://purl.org/np/RAhLBnCkTIy1O1RJGNjrqqWs3d9AifkFB5TOV_4bIv1Ig#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
sp:SeventyFivePercentEthanol
rdfs:label
"75% ethanol" .
sub:_1
rdfs:label
"DNA pellet" .
sub:_2
rdfs:label
"DNA pellet" .
sub:step
dce:description
"Wash DNA pellet by adding 10 ml of 75% ethanol. Centrifuge at 12,000 x g for 10 min, 4 °C" ;
sp:hasExperimentalOutput
sub:_1
;
a
pplan:Step
;
bs:bioSampleUsed
sub:_2
;
bs:reagentUsed
sp:SeventyFivePercentEthanol
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-10T11:23:07.795961"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:JitaRIFWEeu07vs4brmY_A
,
authority:OXwS8IFWEeupVrceUaoIYg
,
authority:VwzrCoFWEeue7A8C-c0wkQ
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"UzMsXuojSL3jftFRjIsCde+4Yv05ToPXhZfNflwXHJKiUsvJ63Nn3t/2RoOWJNP6aEiCG/l7FFE/isDIv7AqQPesa50N6HFI9Vv93wEwUUfoVUe+9dAHVgpszM/DfaXyb7KgsKW08iLz+hxlcnZG7icwmyKmFaOmvI5dUVPIdk8=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-10T11:23:07.795961"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}