@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAkXYXVGmCncQk-W_LGy-TN3MZEfkQYVIs_189GC7evjE> .
@prefix sub: <http://purl.org/np/RAkXYXVGmCncQk-W_LGy-TN3MZEfkQYVIs_189GC7evjE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/OBI_0400155> rdfs:label "water bath" .
  sub:_1 rdfs:label "mycobacterial suspension" .
  sub:_2 rdfs:label "mycobacterial suspension" .
  sub:step dce:description "Incubate mycobacterial suspension at 80 °C for 1 h in a water bath. This step will facilitate loosening of the mycobacterial cell wall and will also inactivate pathogenic mycobacteria." ;
    sp:hasExperimentalInput sub:_1 ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_2 ;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400155> .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-26T09:44:58.004043"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:6fjFjHexEeujQaNQWbt2BA , authority:AF5opHeyEeu2mlcSdJa8dg , authority:EsK33neyEeuHSLu3coEi_g .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "OE2wZd/87EeqHiw7BuAVmfqvvqOHp/7ZqY/4yfMrzG22CMaIT90ck8YojDxtlSdx15iH9QWWHLbr6se6zbd6ISsMNLgmKA6BiVIbPUkvHvNp0PneoNM7HvoJoo0zVcaMfkDlvJfWNkYYqk8JN/FdantfRopnRmcHXHKqn7a+AeQ=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-26T09:44:58.004043"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}