@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAn6Wlx2GAHJOC6lzbBq_nfCgkdhCbtMnyiFfrrEPU2aY
> .
@prefix sub: <
http://purl.org/np/RAn6Wlx2GAHJOC6lzbBq_nfCgkdhCbtMnyiFfrrEPU2aY#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218
>
rdfs:label
"70% ethanol" .
<
http://purl.obolibrary.org/obo/CHEBI_16236
>
rdfs:label
"70% ethanol" .
<
http://purl.obolibrary.org/obo/UBERON_0036018
>
rdfs:label
"pellet " .
sub:_1
rdfs:label
"DNA samples" .
sub:_2
rdfs:label
"DNA samples" .
sub:step
dce:description
" Discard supernatant and wash the pellet by adding 1.8 ml 70% ethanol, vortex thoroughly. At this stage, DNA samples can be stored at room temperature or refrigerated." ;
sp:hasExperimentalOutput
sub:_1
;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/UBERON_0036018
> ,
sub:_2
;
bs:reagentUsed
<
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218
> , <
http://purl.obolibrary.org/obo/CHEBI_16236
> .
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-14T05:48:05.189039"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:OhdXFmBEEeuyUPsQUajIPw
,
authority:YNl9wGBEEeu6UOuSh1WfPQ
,
authority:iJLlpGBEEeuwmCPxCA9Neg
,
authority:rg9crmBEEeu1f2MhpTvsdw
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"W6dDl7O1/rAQSzzWETfpnLkXKgSw66csu9X1ZdG7KhRV2sUnWhyGdo9LucfZjlSST8YfqvaQfE18EaLvucJrAuOXe+R9OfNRfSK2Z52W5+ou/+bReRQlu5vittlTHlhhq25Yfd7ziMRK+intiR/D8VVnRAwV1ckutjtwifLX3xc=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-14T05:48:05.189039"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}