@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RApoqP4lv5AOvQUPlRE1i04QMOITLFL3tQuc6kDT-ZBBI
> .
@prefix sub: <
http://purl.org/np/RApoqP4lv5AOvQUPlRE1i04QMOITLFL3tQuc6kDT-ZBBI#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/OBI_0002488
>
rdfs:label
"1 ml auto pipette" .
<
http://purl.obolibrary.org/obo/UBERON_0000178
>
rdfs:label
"blood" .
sub:_1
rdfs:label
"Ficoll Histopaque" .
sub:step
dce:description
"Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers." ;
sp:hasExperimentalInput
<
http://purl.obolibrary.org/obo/UBERON_0000178
> ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/UBERON_0000178
> ;
bs:labEquipmentUsed
<
http://purl.obolibrary.org/obo/OBI_0002488
> ;
bs:reagentUsed
sub:_1
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-04T15:11:14.099346"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/iannotate
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:SoEpUH0jEeu4hOdUUmy_Fg
,
authority:X1d_-n0jEeuz7d8rRLryUg
,
authority:_N0uzn0iEeuQqqNXdaCtXg
,
authority:ck26An0jEeuIb3dRiLmm0A
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"Sb47s30On9qBTTKZt7/k76cSg1Q1Y/sgBKsQ5gWjN4GOxh27e1eCfWYInndcGdHierLG57hsT9Zntd6KN5kmGrGtCxMvwVGsFoQEtypFXhw5qnVyGaQLPXN69MZiFyDp/CKqn1PTbF7U4esd+/K3Yjg7vlsfqSX9loPgYSme94k=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-04T15:11:14.099346"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/iannotate
> .
}