@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls> .
@prefix sub: <http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  sub:_1 rdfs:label "pellet" .
  sub:_2 rdfs:label "tubes" .
  sub:_3 rdfs:label "TE" .
  sub:step dce:description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." ;
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991> ;
    a pplan:Step ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991> , sub:_1 ;
    bs:labEquipmentUsed sub:_2 ;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> , sub:_3 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-25T16:19:32.955991"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:0vwt1neuEeugLneRnV11IQ , authority:U5V5WHeuEeuaqaeDOnia0g , authority:Zutt3HeuEeuuZH8J3g6HLA , authority:i38vTneuEeuNAsMsRIF24w , authority:oNTjoneuEeu3I3Om5Ihl4A , authority:uGfetneuEeuQLE_Q4_h28w .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "CK0mUuEnSryqDlktLiDzRtTbtqvviyX1sWeq4FLSkqK62YSYfkFnQ/HwTyKezY7DqmGU2q8fUJx2xP1NI/uAPsPyWpMoNuOkKce6F1rlz3waaMHVCHWflyKmCQyXKibwDd0VS/NSBecXujFnqAuLY0mDrXc4VtLm5xIcKrh8YTk=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-25T16:19:32.955991"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}