@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAtKnp_kibv4oYNO4QUDq2a3S7tz2rjOK9Aa2i646ECpY
> .
@prefix sub: <
http://purl.org/np/RAtKnp_kibv4oYNO4QUDq2a3S7tz2rjOK9Aa2i646ECpY#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_16991
>
rdfs:label
"DNA" .
<
http://purl.obolibrary.org/obo/OBI_0000439
>
rdfs:label
"agarose gel" .
<
http://purl.obolibrary.org/obo/OBI_0400115
>
rdfs:label
"spectrophotometer" .
sub:_1
rdfs:label
"pellet" .
sub:_2
rdfs:label
"molecular biology grade water" .
sub:step
dce:description
"Dissolve pellet in molecular biology grade water and analyze DNA by agarose gel electrophoresis and spectrophotometer for its purity and integrity." ;
sp:hasExperimentalOutput
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ,
sub:_1
;
bs:labEquipmentUsed
<
http://purl.obolibrary.org/obo/OBI_0400115
> ;
bs:reagentUsed
<
http://purl.obolibrary.org/obo/OBI_0000439
> ,
sub:_2
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-26T09:39:38.488998"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:Kw3IuHhAEeugvC_qjxSBgA
,
authority:RarDNHg_EeupnnscZLetKg
,
authority:X9K7Ing_EeuO7n-Tus5eYA
,
authority:cBa3cng_EeuBtgsBD4MQRg
,
authority:ml0rzng_EeufVHf7O51lgw
,
authority:r-dENHg_EeuGqktNfgJ-3Q
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"JO31h2KdkoBVPOiK7yGJkS5af3J1kQMQKBNrtFzNUTdM8n5JhJAF8rCt7slhM+bCigR80XflKlNuLlsTnLu4pSEukJH8KTcOkno2jJdnn09RfeS8QurAQ22g+Lk1J7DR8sLu5/nNh/BWxOzk9zpsOMc5wIhEtAzFUQXaHG++oUs=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-26T09:39:38.488998"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}