@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0> .
@prefix sub: <http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/OBI_0002488> rdfs:label "pipette" .
  sub:_1 rdfs:label "mycobacteria" .
  sub:_2 rdfs:label "mycobacteria" .
  sub:_3 rdfs:label "culture medium" .
  sub:_4 rdfs:label "TE" .
  sub:step dce:description """Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette.
Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.""" ;
    sp:hasExperimentalInput sub:_1 ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_2 ;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0002488> ;
    bs:reagentUsed sub:_3 , sub:_4 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-10T11:20:30.406999"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:F5hHDoFQEeupMnfBwWNSFA , authority:dko6BoFPEeuJf99nep5VFA , authority:lwnWrIFPEeuSpOPhPMkNog , authority:vFGivoFPEeu8CqN-Wci9Sw , authority:zppHCoFPEeu7CktybqeS2Q .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "MC6E9m3cxtSsdsvlva8NE1habj8YrPsLAxyw9icRRdO34BiwBHyie47jO85pkdrXP/3ghZ3ziJlfnMN+J/vgmDZToHG9kNd0ZDlCWGJZFurZ3PZCRwBp1TFKiVVs6gjP45/GBml+6vq0wvXyktenXZULNwqu+jADacA7WiyMW+U=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-10T11:20:30.406999"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}