@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ
> .
@prefix sub: <
http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.bioontology.org/ontology/NCBITAXON/2
>
rdfs:label
"bacteria" .
sub:_1
rdfs:label
"pellet " .
sub:_2
rdfs:label
"pestle" .
sub:_3
rdfs:label
"mortar" .
sub:_4
rdfs:label
"liquid N2" .
sub:step
dce:description
"Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it." ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.bioontology.org/ontology/NCBITAXON/2
> ,
sub:_1
;
bs:labEquipmentUsed
sub:_2
,
sub:_3
;
bs:reagentUsed
sub:_4
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-10T11:13:12.232860"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:5T5BtoFSEeuDVNszA62k8g
,
authority:SsTfhoFTEeugz7M1dQr42w
,
authority:XnU0XoFTEeu03pMhdlRcng
,
authority:bKJEkIFTEeu4IH819XJVFw
,
authority:fN60OIFTEeuXvTMD-qL4vw
,
authority:mK8Z8IFTEeuvOSvYxUpUaw
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"N4l+SnKO+1ioBbTttjSPHif+OJ3qBq7H/5m+yE5MQuX7xASSwc+U079Awu1kCXlNTooib3QoI5pdi/kklyNs4yFWOd6Ly8//iRnqNuf+44qRPezHNr8YJGSdX/JMKa3ZYrZ23cMju+zeWDT8+RQJwKpb8TtX27wbCQJbrJrHg+U=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-10T11:13:12.232860"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}