@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAwHKkQ8dmPkzhTGOQN0lPMrHLzjbm-73-rXPXrb-vtEY> .
@prefix sub: <http://purl.org/np/RAwHKkQ8dmPkzhTGOQN0lPMrHLzjbm-73-rXPXrb-vtEY#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "tubes" .
  sub:_2 rdfs:label "swing-out bucket" .
  sub:step dc:description "Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase." ;
    a pplan:Step ;
    bs:labEquipmentUsed sub:_1 , sub:_2 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-04T15:10:39.161366"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:hK6GzH0jEeueOYfjmWjLNw , authority:msaalH0jEeuljtOq3z64Ug , authority:sCWovH0jEeuMj2caW7AzRg .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "WMHnj4yhxOU1dzFkidCo2H6K/LwByfjbpX0OqBZZRqFpEAzzTKZJIOjf7Z73FQcqqjman3rM+9BZwsJyUW5DhQR1lrVxY0mb8QD89hXq4CispCSrDFHq2cgvP2T0nJ5Hy0JCj+dhB+ayX39d95v0R1OCUX91cD1mZjJ5KbopLME=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-04T15:10:39.161366"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}