@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAyJ6cwDL8iRxGaDg6PeZ-QMjwzFN99hnEQsC08H2yhaA
> .
@prefix sub: <
http://purl.org/np/RAyJ6cwDL8iRxGaDg6PeZ-QMjwzFN99hnEQsC08H2yhaA#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix dc: <
http://purl.org/dc/elements/1.1/
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/OBI_0000918
>
rdfs:label
"Dulbecco's modified eagle medium" .
<
http://purl.obolibrary.org/obo/OBI_1000024
>
rdfs:label
"cell pellet" .
sub:_1
rdfs:label
"medium" .
sub:step
dc:description
"Discard medium and re-suspend the cell pellet in 1 ml of sterile Dulbecco's modified eagle medium." ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/OBI_1000024
> ;
bs:reagentUsed
<
http://purl.obolibrary.org/obo/OBI_0000918
> ,
sub:_1
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-14T05:27:16.013336"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:IXNLMG6tEeuzfOcdUB4Nvg
,
authority:MR2YGm6tEeua50dSALniKg
,
authority:StYJpG6tEeugDptRWDXrUg
,
authority:X5uYhm6tEeu5occrY5SXuA
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"fsx+RoXFRllp/9pRs7z0vXMsqMpqVpymApEo0biUHK9yj2NYEX6q/S1U0UK8vm/Xkp0P2cia8LPmvamakk/IQki5lnMdl9uLD8OQxDNeFThauegejnSLHR6y6BogNKHShZX6u5TIj6r9h1PkNMsQyqJk96C31Br0dScRSiQ8LVU=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-14T05:27:16.013336"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}